Канал: CurrentProtocols

Example of a typical calcium imaging recording in cerebral organoids

Example of a typical calcium imaging recording in cerebral organoids

Tracking cell growth by using IncuCyte

Tracking cell growth by using IncuCyte

A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: measure blastema area

A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: measure blastema area

A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: measure planarian day 1

A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: measure planarian day 1

A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: scale an image in ImageJ

A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: scale an image in ImageJ

Murine-Derived Glioma Organoids and Cell Line Culture Systems

Murine-Derived Glioma Organoids and Cell Line Culture Systems

The 21st Annual Wiley Prize in Biomedical Sciences

The 21st Annual Wiley Prize in Biomedical Sciences

Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 2)

Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 2)

Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 1)

Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 1)

View of contracting sarcomeres in hiPSC-CMs expressing ACTN2-GFP and the SotaTool output graphs

View of contracting sarcomeres in hiPSC-CMs expressing ACTN2-GFP and the SotaTool output graphs

3D image of autofluorescent mouse cochlea

3D image of autofluorescent mouse cochlea

Indicator of a breathing mouse

Indicator of a breathing mouse

Checking for leakage with deep inflation perturbation

Checking for leakage with deep inflation perturbation

Measuring lung function of unchallenged mice

Measuring lung function of unchallenged mice

Demonstration of CUT&RUN washes

Demonstration of CUT&RUN washes

The 20th Annual Wiley Prize in Biomedical Sciences

The 20th Annual Wiley Prize in Biomedical Sciences

Assessment of Data Quality Using Expert Gating and Dimensionality Reduction Algorithms

Assessment of Data Quality Using Expert Gating and Dimensionality Reduction Algorithms

Heterogeneous Autofluorescence

Heterogeneous Autofluorescence

Deciding on an Approach for Mitigating Autofluorescence

Deciding on an Approach for Mitigating Autofluorescence

Evaluation of Marker Resolution

Evaluation of Marker Resolution

Unmixing Evaluation of Fully Stained Sample

Unmixing Evaluation of Fully Stained Sample

Evaluation of Optimal Spectral Reference Controls

Evaluation of Optimal Spectral Reference Controls

A Simple Method for Creating a High-content Microscope for Imaging Multiplexed Tissue Microarrays

A Simple Method for Creating a High-content Microscope for Imaging Multiplexed Tissue Microarrays

Fabrication and Characterization of Drug-Releasing Microspheres

Fabrication and Characterization of Drug-Releasing Microspheres

Normal Rodent Step Cycle in CatWalk XT Software

Normal Rodent Step Cycle in CatWalk XT Software

Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds

Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds

Preparing Ionically Crosslinked Alginate Hydrogels

Preparing Ionically Crosslinked Alginate Hydrogels

Obtaining Eggs from Xenopus Laevis Females

Obtaining Eggs from Xenopus Laevis Females

Preparation and Fractionation of Xenopus Laevis Egg Extracts

Preparation and Fractionation of Xenopus Laevis Egg Extracts

Cell DNA and FISH Analysis for Application to Preimplantation Genetic Diagnosis

Cell DNA and FISH Analysis for Application to Preimplantation Genetic Diagnosis

Dissecting Animal Cap Explants from Frog Embryos

Dissecting Animal Cap Explants from Frog Embryos

Purification of Intact Chloroplasts

Purification of Intact Chloroplasts

Preparing a Stainless Steel Loop for Manipulation of Grids

Preparing a Stainless Steel Loop for Manipulation of Grids

Labeling and Washing Grid-Mounted Section

Labeling and Washing Grid-Mounted Section

Spontaneously Beating hESC-EBs on Culture Day 8

Spontaneously Beating hESC-EBs on Culture Day 8

Chromosomal Stretching Experiment

Chromosomal Stretching Experiment

Measuring the Spring Constant of a Micropipet (Support Protocol 6).

Measuring the Spring Constant of a Micropipet (Support Protocol 6).

Calibration of the Needle with Masses (Support Protocol 7).

Calibration of the Needle with Masses (Support Protocol 7).

Pre-subtraction Dataset Acquired for BaLM Analysis.

Pre-subtraction Dataset Acquired for BaLM Analysis.

Whole-Embryo Development from HH3 to HH9 Over a Period of 24 Hours

Whole-Embryo Development from HH3 to HH9 Over a Period of 24 Hours

Example of Mitochondrial Movement in a Distal Axon Segment.

Example of Mitochondrial Movement in a Distal Axon Segment.

In Vitro Culturing and Screening of Candida albicans Biofilms (Pt. 2)

In Vitro Culturing and Screening of Candida albicans Biofilms (Pt. 2)

In Vitro Culturing and Screening of Candida albicans Biofilms (Pt. 1)

In Vitro Culturing and Screening of Candida albicans Biofilms (Pt. 1)

Time-lapse imaging of tangentially migrating neuroblasts in the adult RMS.

Time-lapse imaging of tangentially migrating neuroblasts in the adult RMS.

Appearance of a freshly passaged (within 16 to 40 hr) liquid culture of H. pylori strain SS-1

Appearance of a freshly passaged (within 16 to 40 hr) liquid culture of H. pylori strain SS-1

An older (3 to 4 days after passage) liquid culture of H. pylori strain SS-1

An older (3 to 4 days after passage) liquid culture of H. pylori strain SS-1

3D Molecular Tomography with CNIR5

3D Molecular Tomography with CNIR5

3D Molecular Tomography with tdTomato

3D Molecular Tomography with tdTomato

Basic manipulations of Brachyspira species

Basic manipulations of Brachyspira species

Assembly of Synapses pt. 1

Assembly of Synapses pt. 1

Assembly of Synapses pt. 2

Assembly of Synapses pt. 2

High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy

High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy

Signal Sampling and Aliasing

Signal Sampling and Aliasing

Pin Loading

Pin Loading

Slide Loading

Slide Loading

Initiate Printing

Initiate Printing

Spectral Imaging Methods Compared

Spectral Imaging Methods Compared

Grating Orders

Grating Orders

Liveimaging of a Growing Enteroid

Liveimaging of a Growing Enteroid

Enteroid Expressing RFP-tagged ZO1

Enteroid Expressing RFP-tagged ZO1

Bright-field and RFP-Tagged ZO1 Overlay During Live Imaging of a Growing Enteroid

Bright-field and RFP-Tagged ZO1 Overlay During Live Imaging of a Growing Enteroid

Abdomen of a mouse with genetically induced pancreatic carcinoma by multimodal PET-CT

Abdomen of a mouse with genetically induced pancreatic carcinoma by multimodal PET-CT

3D Rendering of a mouse with genetically induced disseminated melanoma by PET-CT

3D Rendering of a mouse with genetically induced disseminated melanoma by PET-CT

3D Rendering of a Tumor-Free Mouse by PET-CT

3D Rendering of a Tumor-Free Mouse by PET-CT

Live Imaging of the Lung pt. 2

Live Imaging of the Lung pt. 2

Live Imaging of the Lung pt. 1

Live Imaging of the Lung pt. 1

3-D Neuron and Neurite Reconstruction for Rotenone-treated Neurons

3-D Neuron and Neurite Reconstruction for Rotenone-treated Neurons

Reconstructed 3-D Image Stacks Using a Skeletonization Algorithm

Reconstructed 3-D Image Stacks Using a Skeletonization Algorithm

Motorized Stage Instruction Movie

Motorized Stage Instruction Movie

Enteroid Crypt Domain Endogenously Expressing ZO1-RFP and Immunostained for E-cadherin

Enteroid Crypt Domain Endogenously Expressing ZO1-RFP and Immunostained for E-cadherin

Liveimaging of a Growing Enteroid Endogenously Expressing Cytosolic YFP

Liveimaging of a Growing Enteroid Endogenously Expressing Cytosolic YFP

Extrusion of Dead Cells Contained in the Lumen to Exterior

Extrusion of Dead Cells Contained in the Lumen to Exterior

Hepatocytes and Rhythmic Bioluminescent Expression of PER2::LUC

Hepatocytes and Rhythmic Bioluminescent Expression of PER2::LUC

Stem cell Containing Crypt Domain Regenerates and Seals to Stay Separated from Damaged Region

Stem cell Containing Crypt Domain Regenerates and Seals to Stay Separated from Damaged Region

Hepatocytes Flatten and Establish Contact with Other Cells in the First 24 hr After Plating

Hepatocytes Flatten and Establish Contact with Other Cells in the First 24 hr After Plating

Dissecting Animal Cap Explants from Frog Embryos

Dissecting Animal Cap Explants from Frog Embryos

Removing the Jelly Coat from Frog Eggs

Removing the Jelly Coat from Frog Eggs

In Vitro-Induced Beating Heart

In Vitro-Induced Beating Heart

Movement of the Shaker for Fixation of Animal Caps

Movement of the Shaker for Fixation of Animal Caps

Movement of the Shaker for Whole-Mount in Situ Hybridization

Movement of the Shaker for Whole-Mount in Situ Hybridization

Elongation Movement of an Activin-Treated Animal Cap

Elongation Movement of an Activin-Treated Animal Cap

Two-Photon Video Microscopy of Embryonic Dividing NBs

Two-Photon Video Microscopy of Embryonic Dividing NBs

Time-Lapse Micrograph of the Last 24hr of Branching and Maturation of hPSC-derived 3D Lung Organoids

Time-Lapse Micrograph of the Last 24hr of Branching and Maturation of hPSC-derived 3D Lung Organoids

Lung Organoids Come to Contain Smooth Muscle

Lung Organoids Come to Contain Smooth Muscle

Emergence of iPSC Colonies

Emergence of iPSC Colonies

A Simple Method for Creating a High-Content Microscope for Imaging Multiplexed Tissue Microarrays

A Simple Method for Creating a High-Content Microscope for Imaging Multiplexed Tissue Microarrays

Creating Cytocentrifuge Unit Tops

Creating Cytocentrifuge Unit Tops

Creating Cytocentrifuge Unit Bases

Creating Cytocentrifuge Unit Bases

Target Cell Suspension Density

Target Cell Suspension Density

Assembling Cytocentrifuge Units

Assembling Cytocentrifuge Units

Diassembling Cytocentrifuge Units

Diassembling Cytocentrifuge Units

Mounting Coverslips

Mounting Coverslips

Preparing Ionically Crosslinked Alganate Hydrogels

Preparing Ionically Crosslinked Alganate Hydrogels

Aminosilane Slide Coating

Aminosilane Slide Coating

Detection of Array-Immobilized DNA

Detection of Array-Immobilized DNA

Slide Expression, Detection, and Imaging

Slide Expression, Detection, and Imaging

Preparation of Master Mix and the Arrayer

Preparation of Master Mix and the Arrayer

DNA Isolation and Array Sample Preparation

DNA Isolation and Array Sample Preparation

Method for the Production of Aqueous Cigarette Smoke Extract.

Method for the Production of Aqueous Cigarette Smoke Extract.

Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds

Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds

Fully Automated Ancestral Sequence Reconstruction using FireProtASR

Fully Automated Ancestral Sequence Reconstruction using FireProtASR

Fast Relaxation Imaging of PGK Folding in a U2OS Cell

Fast Relaxation Imaging of PGK Folding in a U2OS Cell

Labeling Dynamic Microtubule Ends (Pt. 1)

Labeling Dynamic Microtubule Ends (Pt. 1)

Labeling Dynamic Microtubule Ends (Pt. 2)

Labeling Dynamic Microtubule Ends (Pt. 2)

Calcium Spike in Root Tissue Upon Application of AtRALF1

Calcium Spike in Root Tissue Upon Application of AtRALF1

Shoot Apical Meristem (SAM) Laser Microdissection

Shoot Apical Meristem (SAM) Laser Microdissection

Principles of Aseptic Technique in Mammalian Cell Culture

Principles of Aseptic Technique in Mammalian Cell Culture

Day 30 Post-Differentiation hiPSC-CMs Exhibiting an N-terminus titin-GFP Fusion Tag

Day 30 Post-Differentiation hiPSC-CMs Exhibiting an N-terminus titin-GFP Fusion Tag

Assembly of an Alternative Feeding Chamber for Aphids

Assembly of an Alternative Feeding Chamber for Aphids

Assembly of a Feeding Chamber for Aphids

Assembly of a Feeding Chamber for Aphids

UVB Triggers Secretion of VSVG‐YFP‐2xUVR8 in a COS7 Cell

UVB Triggers Secretion of VSVG‐YFP‐2xUVR8 in a COS7 Cell

Example of Mitochondrial Movement in a Cos-7 Cell

Example of Mitochondrial Movement in a Cos-7 Cell

Glycolytic Oscillations in Individual Yeast Cells

Glycolytic Oscillations in Individual Yeast Cells

Bonding of the Microfluidic Device

Bonding of the Microfluidic Device

Comparative View of a BaLM Dataset Before and After Subtraction

Comparative View of a BaLM Dataset Before and After Subtraction

Harnessing neoantigens for individualized cancer immunotherapy

Harnessing neoantigens for individualized cancer immunotherapy

A Multi-center Study Evaluation of the ClearLLab 10C Panels

A Multi-center Study Evaluation of the ClearLLab 10C Panels

ImageStream and the challenge of distinguishing cell-cell complexes from singlet cells in FC

ImageStream and the challenge of distinguishing cell-cell complexes from singlet cells in FC

Introducing the new 13 colour CE IVD flow cytometer for both the routine and clinical research labor

Introducing the new 13 colour CE IVD flow cytometer for both the routine and clinical research labor

Mapping dynamic immunity over time, space and disease.

Mapping dynamic immunity over time, space and disease.

Operating an Imaging Core Facility During a Pandemic

Operating an Imaging Core Facility During a Pandemic

Providing a Solid Foundation for Simpler and Smarter Flow Cytometry Panel Design

Providing a Solid Foundation for Simpler and Smarter Flow Cytometry Panel Design

Containment of DNA by HEPA filters to reduce potential DNA contamination in experiments

Containment of DNA by HEPA filters to reduce potential DNA contamination in experiments

Extracellular Vesicle Detection

Extracellular Vesicle Detection

Choosing Labels and Conjugating Antibodies for Multicolor Flow Cytometry and Other Immunoassays

Choosing Labels and Conjugating Antibodies for Multicolor Flow Cytometry and Other Immunoassays

Applications in CAR T-Cell Therapy: : Dissecting Cellular Composition Using Single Cell Multiomics.

Applications in CAR T-Cell Therapy: : Dissecting Cellular Composition Using Single Cell Multiomics.

Extracellular Vesicle Enrichment and Characterization from Cell Culture Supernatants and Biological

Extracellular Vesicle Enrichment and Characterization from Cell Culture Supernatants and Biological

Atypical Femur Fractures: Addressing the Problem from a Patient-Centered Perspective

Atypical Femur Fractures: Addressing the Problem from a Patient-Centered Perspective

Combining Data Independent Analysis for Broad Scale Phenotyping

Combining Data Independent Analysis for Broad Scale Phenotyping

Cancer Stem Cells, Hypoxia and Tumor Treatment Response

Cancer Stem Cells, Hypoxia and Tumor Treatment Response

Cancer Stem Cells and Mechanisms of Multi Drug Resistance by Flow Cytometry

Cancer Stem Cells and Mechanisms of Multi Drug Resistance by Flow Cytometry

A New CE IVD Orientation Tube to Guide You Through Diagnosis of Hematolymphoid Malignancies

A New CE IVD Orientation Tube to Guide You Through Diagnosis of Hematolymphoid Malignancies

Evaluating expression patterns of multiple inhibitory and stimulatory receptors on NK cells

Evaluating expression patterns of multiple inhibitory and stimulatory receptors on NK cells

Neuroinflammation Models Use in Studies of Neurodegeneration and Regeneration

Neuroinflammation Models Use in Studies of Neurodegeneration and Regeneration

Making Panel Design Easier in the Era of High Dimensional Flow Cytometry

Making Panel Design Easier in the Era of High Dimensional Flow Cytometry

Resolving Human Circulating Innate Lymphoid Cell Heterogeneity Using Advanced Single Cell Analysis T

Resolving Human Circulating Innate Lymphoid Cell Heterogeneity Using Advanced Single Cell Analysis T

Measuring Toxicity Biomarkers in Mouse Models and Other Small volume Samples

Measuring Toxicity Biomarkers in Mouse Models and Other Small volume Samples

Overcoming Sample related Challenges for Elispot and Other Functional T cell Assays To Produce More

Overcoming Sample related Challenges for Elispot and Other Functional T cell Assays To Produce More

Multiplexing MS Identification/Quantification of Site-Specific Reversible Cysteine Modification

Multiplexing MS Identification/Quantification of Site-Specific Reversible Cysteine Modification

The 17th Annual Wiley Prize in Biomedical Sciences

The 17th Annual Wiley Prize in Biomedical Sciences

Generation and Use of iPSC derived Microglia to Study Neurodegenerative Diseases

Generation and Use of iPSC derived Microglia to Study Neurodegenerative Diseases

Photodetection in Flow Cytometry

Photodetection in Flow Cytometry

The ABCs of ADC characterisation

The ABCs of ADC characterisation

Increasing Large Scale Protein Production Using a Novel Supplement

Increasing Large Scale Protein Production Using a Novel Supplement

The Complex Pharmacology of T Cell CARs

The Complex Pharmacology of T Cell CARs

The 16th Annual Wiley Prize in Biomedical Sciences

The 16th Annual Wiley Prize in Biomedical Sciences

Methods for Validating a Gene Editing Approach to Treating Brain Disease.

Methods for Validating a Gene Editing Approach to Treating Brain Disease.

Simplified Methods for Investigating Deregulation of the Cell Cycle and Apoptosis in Cancer Progress

Simplified Methods for Investigating Deregulation of the Cell Cycle and Apoptosis in Cancer Progress

High Throughput Antibody Discovery Targeting Glycoproteins for Apoptosis Induction in Cancer

High Throughput Antibody Discovery Targeting Glycoproteins for Apoptosis Induction in Cancer

The 14th Annual Wiley Prize in Biomedical Sciences

The 14th Annual Wiley Prize in Biomedical Sciences

How Mycoplasma Contamination Affects Results and Reproducibility

How Mycoplasma Contamination Affects Results and Reproducibility

Single Cell Analysis Getting More From Your Data

Single Cell Analysis Getting More From Your Data

Serum Alternatives for Adult Stem Cell Expansion for Allogeneic and Autologous Therapies

Serum Alternatives for Adult Stem Cell Expansion for Allogeneic and Autologous Therapies

Single Cell Analysis Unveiling Tumor Heterogeneity

Single Cell Analysis Unveiling Tumor Heterogeneity

More parameters, more informative phenotypes: Systematic phenotyping approaches

More parameters, more informative phenotypes: Systematic phenotyping approaches

Plant Genome Editing and Engineering

Plant Genome Editing and Engineering

The Race Is On: Faster Turnaround Times in the Diagnosis of Multidrug Resistant Tuberculosis

The Race Is On: Faster Turnaround Times in the Diagnosis of Multidrug Resistant Tuberculosis

The 18th Annual Wiley Prize in Biomedical Sciences

The 18th Annual Wiley Prize in Biomedical Sciences

Maximizing High Dimensional Human Immune Monitoring with Mass Cytometry

Maximizing High Dimensional Human Immune Monitoring with Mass Cytometry

High Dimensional Human Immune Profiling Simplified

High Dimensional Human Immune Profiling Simplified

Initial Evaluation of the ClearLLab10C System in the Immunophenotyping of Leukemia

Initial Evaluation of the ClearLLab10C System in the Immunophenotyping of Leukemia

The 15th Annual Wiley Prize in Biomedical Sciences

The 15th Annual Wiley Prize in Biomedical Sciences

Using microCT to Assess Bone Loss in Preclinical Models

Using microCT to Assess Bone Loss in Preclinical Models

The 19th Annual Wiley Prize in Biomedical Sciences

The 19th Annual Wiley Prize in Biomedical Sciences

The Role of GC-MS/MS in Metabolomics Experiments and the Exploration of Optimized Workflows

The Role of GC-MS/MS in Metabolomics Experiments and the Exploration of Optimized Workflows

Metabolomic Profiling of Anionic Metabolites in Head and Neck Cancer Cells

Metabolomic Profiling of Anionic Metabolites in Head and Neck Cancer Cells

A Translational Proteomics Workflow Coupling Immunoenrichment to MSIA-SRM & its Application to AD

A Translational Proteomics Workflow Coupling Immunoenrichment to MSIA-SRM & its Application to AD

Multiplexed Protein Detection Assay Fundamentals and Applications in Cancer Research

Multiplexed Protein Detection Assay Fundamentals and Applications in Cancer Research

Stem Cell and Reprogramming Research Methods

Stem Cell and Reprogramming Research Methods

LC-MS-based Metabolomics: Workflows, Strategies and Challenges

LC-MS-based Metabolomics: Workflows, Strategies and Challenges

Flow Cytometry: Essential Instrument and Experimental Design Considerations

Flow Cytometry: Essential Instrument and Experimental Design Considerations

Advances in Protein Detection and Quantitation Methods

Advances in Protein Detection and Quantitation Methods

Microfabrication and Assembly of the Microfluidic Perfusion Device

Microfabrication and Assembly of the Microfluidic Perfusion Device

The 2012 Wiley Symposium

The 2012 Wiley Symposium

Contracting Human EHT to Facilitate Auxotonic Contractions

Contracting Human EHT to Facilitate Auxotonic Contractions

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

Staining Proteins in Gels

Staining Proteins in Gels

UPDRS Motor Subscale 3, First Half

UPDRS Motor Subscale 3, First Half

UPDRS Motor Subscale 3, Second Half

UPDRS Motor Subscale 3, Second Half

Technique for Putting on Surgical Gloves

Technique for Putting on Surgical Gloves

Ultrathin Section Retrieval and Transfer to Formvar-coated grids

Ultrathin Section Retrieval and Transfer to Formvar-coated grids

Preparing a Mounted Eyelash for Handling Sections

Preparing a Mounted Eyelash for Handling Sections

Sucrose Infiltration of Tissues/cells Fixed for Cryo-immunogold Labeling

Sucrose Infiltration of Tissues/cells Fixed for Cryo-immunogold Labeling

Plunge Freezing of Tissue/cells for Cryo-Immunogold Labeling

Plunge Freezing of Tissue/cells for Cryo-Immunogold Labeling

Fixation of Cells for Cryo-immunogold Labeling

Fixation of Cells for Cryo-immunogold Labeling

Application of Silicone Grease

Application of Silicone Grease

Assembly of a Trichamber

Assembly of a Trichamber

Coating Grids With Formvar

Coating Grids With Formvar

Major Components of the Light Microscope

Major Components of the Light Microscope

Freezing Thawing and Packaging Cells for Transport

Freezing Thawing and Packaging Cells for Transport

Electrophoretic Separation of Proteins

Electrophoretic Separation of Proteins

Counting and Determining the Viability of Cultured Cells

Counting and Determining the Viability of Cultured Cells

Phase Contrast and Differential Interference Contrast DIC Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Proper Care and Cleaning of the Microscope

Proper Care and Cleaning of the Microscope

Ultrathin Cryo Sectioning

Ultrathin Cryo Sectioning