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Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis - a 2 minute Preview of the Experimental Protocol

Katarzyna Okurowska, Sanhita Roy, Praveen Thokala, Lynda Partridge, Prashant Garg, Sheila MacNeil, Peter N. Monk, Esther Karunakaran
University of Sheffield, Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB); University of Sheffield, Department of Chemical and Biological Engineering; L V Prasad Eye Institute, Hyderabad Eye Research Foundation; University of Sheffield, Health Economics and Decision Science, School of Health and Related Research; University of Sheffield, Department of Molecular Biology and Biotechnology; University of Sheffield, Department of Materials Science and Engineering; University of Sheffield, Department of Infection, Immunity and Cardiovascular Disease;

This article describes a step-by-step protocol to set up an ex vivo porcine model of bacterial keratitis. Pseudomonas aeruginosa is used as a prototypic organism. This innovative model mimics in vivo infection as bacterial proliferation is dependent on the ability of the bacterium to damage corneal tissue.

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