Scrambled eggs from the frog Xenopus laevis retain more life than expected. They spontaneously form cell-like compartments and these compartments can divide periodically. (See the research paper by Xianrui Cheng and James Ferrell in Science 366, 631-637, 2019, link shown below)
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This video shows cell-like compartments undergoing five consecutive cell division cycles in a sperm-supplemented cycling egg extract. In this experiment, the daughter compartments formed after every division contained a single nucleus each. Bright-field images are shown in gray, and mCherry-NLS signal, which visualizes interphase nuclei, is shown in red.
Check out how these cell-like compartments spontaneously arise from scrambled eggs:
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To see videos of this phenomenon with more fluorescent colors and probes, check out these:
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See this phenomenon by eye:
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Check out more research on my website:
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Additional background information:
The extract is made from activated Xenopus laevis eggs (see Andrew Murray, Methods in Cell Biology Volume 36, 1991, Pages 581-605). They can carry out cell cycles autonomously without additional special treatment, because the cell cycle regulator protein cyclin B1 is synthesized and degraded periodically driven by a robust biochemical oscillator consisting of many other proteins. The extract is deposited on a plastic Petri dish, and covered with oil before commencing imaging.
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