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Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes - a 2 minute Preview of the Experimental Protocol

Alexander Goedel, Dorota M. Zawada, Fangfang Zhang, Zhifen Chen, Alessandra Moretti, Daniel Sinnecker
Technical University of Munich, Medical Department I, University Hospital Klinikum rechts der Isar; German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance,; Harvard Medical School, Beth Israel Deaconess Medical Center;

Here we present a method to optically image action potentials, specifically in ventricular-like induced pluripotent stem cell-derived cardiomyocytes. The method is based on the promoter-driven expression of a voltage-sensitive fluorescent protein.

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