Light Scattering Techniques
Speaker: Chris Johnson, MRC Laboratory of Molecular Biology, UK

The LMB Biophysics Facility houses a wide range of state-of-the-art and in-house built instruments that enable the molecular characterisation of macromolecular size, shape, mass, stability, binding affinity, dynamics and interaction kinetics.

The facility is primarily intended for hands-on research by students, postdocs, and group leaders, who prepare samples and make measurements. Biophysics supports users in advice on experimental techniques and results, training and in collaborative research.

In this video Chris explains physical principles that underlie light scattering measurements. He describes in depth how size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) is used to determine molar mass in solution, using examples of experiments preformed in the LMB using proteins, complexes and detergent micelles. Additionally, he shows how conjugate analysis can determine the extent of modification of proteins such as glycosylation. Finally, it is shown how comparison of different light-scattering signals can give information on protein shape.


The Biophysics Facility is headed by Chris Johnson, supported by Stephen McLaughlin. Read more about the facility here: [ Ссылка ]

You can find more videos from the LMB 2022 Biophysics Techniques Lecture series here: [ Ссылка ]

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About the MRC Laboratory of Molecular Biology (LMB):
The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.

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