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Three-Dimensional (3D) Tumor Spheroid Invasion Assay - a 2 minute Preview of the Experimental Protocol

Maria Vinci, Carol Box, Suzanne A. Eccles
The Institute of Cancer Research, Division of Molecular Pathology; The Institute of Cancer Research, Division of Cancer Therapeutics;

Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.

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