Watch the Full Video at [ Ссылка ].

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks - a 2 minute Preview of the Experimental Protocol

Shiv Poojan, Han-Seong Kim, Ji-Woon Yoon, Hye Won Sim, Kyeong-Man Hong
National Cancer Center, Omics Core Lab, Research Institute; Inje University Ilsan Paik Hospital, Department of Pathology;

Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.

Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel: [ Ссылка ]

свернуть