Clear localization of two or more target antigens on a tissue section offers investigators deep insights about gene expression, spatial relationships, and disease states. Achieving reliable and meaningful multiple antigen staining on the same section, often referred to as multiplexing, involves careful selection of the detection reagents to avoid interference between the labels and ambiguous results. This webinar will help investigators looking to establish multiplex immunohistochemistry (IHC) in their lab and investigators looking to obtain consistency with the approach.
Topics covered include:
• Getting started with multiplex IHC
• Reagent selection to avoid cross-reactivity
• Appropriate controls
• Counterstaining and co-localization considerations
