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A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids - a 2 minute Preview of the Experimental Protocol

Jorik M. van Rijn, Marliek van Hoesel, Sabine Middendorp
University Medical Center Utrecht, Utrecht University, Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children's Hospital; University Medical Center Utrecht, Utrecht University, Regenerative Medicine Center; Uppsala University, Science for Life Laboratory, Department of Medical Biochemistry and Microbiology;

This protocol describes an assay for the characterization of lipid droplet (LD) formation in human intestinal organoids upon stimulation with fatty acids. We discuss how this assay is used for quantification of LD formation, and how it can be used for high throughput screening for drugs that affect LD formation.

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