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Processing the Loblolly Pine PtGen2 cDNA Microarray - a 2 minute Preview of the Experimental Protocol
W. Walter Lorenz, Yuan-Sheng Yu, Marta Simões, Jeffrey F. D. Dean
University of Georgia (UGA), Warnell School of Forestry and Natural Resources; Instituto Tecnologia Química e Biológica UNL, Av. da República, Instituto de Biologia Experimental e Tecnológica;
The cDNA microarray PtGen2 was developed for gene expression studies in loblolly pine, P. taeda, and other conifer species. Here, we show pre- and post-hybridization handling and washing techniques that can be used with this array to yield better consistency, reduced artifacts, and lower backgrounds.
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