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This tutorial video introduces one of my favorite lab techniques, western blotting. Western blot technique is used to isolate, visualize, and study proteins. This is the 1st part of a series of western blot technique videos I will be uploading.
Time stamps
0:00 Introduction to western blot technique
5:27 Materials for lysing cells
7:10 Washing cells with 1XTBS
8:40 Aspirating TBS (washing buffer) completely
10:04 Adding lysing buffer (RIPA)
10:51 Front view of lysing cells with scraper
11:40 Top view of lysing cells with scraper
13:00 Relaxing side view of lysing cells with lofi music
18:35 Centrifuging cell lysates
19:08 Collecting cell supernatant without the cell pellet
21:43 Preparing protein samples for BCA, DF=1/3
25:36 2nd example of preparing protein samples for BCA, DF=1/4
28:11 BCA materials and protocol
30:35 Pipetting protein standards and samples into 96-well plate for BCA
39:10 Calculations to determine amounts of reagents A and B
42:20 Top view or pipetting 200uL of reagents A and B mixture
43:28 Close up view of pipetting 200uL of reagents A and B mixture
44:03 Shaking 96-well plate for incubation at 37C
