Poster presentation of Felipe Cybis Pereira, PhD student at Physics for Medicine laboratory and Iconeus, at fUSbrain 2022 conference.
"Functional Ultrasound (fUS) imaging has recently been demonstrated to robustly record brain-wide cerebral blood volume (CBV) dynamics as an indirect measure of neural activity over several weeks or months in freely moving rodents1, 2.
In order to bring it forward as routine technique in the field of behavioural neurosciences, the aim of this work was to implement a reliable and robust methodological and technological pipeline from surgery to unrestrained animal movement and finally to data analysis. The presented work focuses on behaviour-CBV data synchronisation and data analysis for spatial navigation, a well-studied behaviour in rodents, as well as insights on atlas registration and probe placement for freely moving fUS.
[1] Sieu, Lim Anna, et al. 2015. Nature Methods 12, no. 9 (August): 831–834. doi:10.1038/nmeth.3506.
[2] Bergel, Antoine, et al. 2020. Nature Communications 11, no. 1 (December). doi:10.1038/s41467-020-19948-7"
