Watch the Full Video at [ Ссылка ].
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System - a 2 minute Preview of the Experimental Protocol
Shenglan Li, Haipeng Xue, Bo Long, Li Sun, Tai Truong, Ying Liu
The University of Texas Health Science Center at Houston, Department of Neurosurgery; The University of Texas Health Science Center at Houston, Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine; The University of Texas Health Science Center at Houston, The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine; The University of Texas Health Science Center at Houston, Summer Research Program, Office of Educational Programs; Shengjing Hospital, China Medical University, Department of Anesthesiology; Shanghai Jiaotong University School of Medicine, Department of Oncology, Renji Hospital; University of West Georgia, Biology Department;
Genome editing tools such as the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system have greatly improved gene targeting efficiency in human induced pluripotent stem cells (hiPSCs). This manuscript describes a protocol for generating lineage specific hiPSC reporter using CRISPR/Cas system assisted homologous recombination.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: [ Ссылка ]
