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A System for ex vivo Culturing of Embryonic Pancreas - a 2 minute Preview of the Experimental Protocol
Kristin M. Petzold, Francesca M. Spagnoli
Max-Delbrück-Center for Molecular Medicine, Molecular and Cellular Basis of Embryonic Development;
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
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