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A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles - a 2 minute Preview of the Experimental Protocol

Huan Bao, Franck Duong, Catherine S. Chan
University of British Columbia , Department of Biochemistry and Molecular Biology;

Nanodiscs are small discoid particles that incorporate membrane proteins into a small patch of phospholipid bilayer. We provide a visual protocol that shows the step-by-step incorporation of the MalFGK2 transporter into a disc.

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