Stem cell therapy describes when stem cells are used for the prevention or treatment diseases such as stroke, Alzheimer’s disease, Parkinson’s disease, spinal cord injury, and other various neurological conditions. Fibroblasts are an active cell component of connective tissue which aid in the maintenance of tissue metabolism. Mouse embryonic fibroblasts (MEF) secrete growth factors and provide a cellular matrix for the nourishment and growth support of embryonic stem cells. As embryonic stem cells are a staple technique in cell and molecular biology research, mouse fibroblast cell lines are critical for the stem cells’ proper growth. Stem cells are also highly susceptible to environmental factors and extra care must be taken when handling them. Using high-efficiency transfection reagents, such as those provided by Altogen Biosystems, allows for greater experimental reproducibility and lower cytotoxicity. The BALB/3T3 cell line was first established in 1968 from BALB/c mouse embryo fibroblast tissue. The BALB/3T3 cell model is a suitable transfection host and is used for the study of mammalian embryonic tissue.
Altogen Biosystems offers pre-optimized biodegradable polymer based BALB/3T3 transfection kits available at:
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