Altogen Biosystems [ Ссылка ] provides the U87 Transfection Reagent for glioblastoma cells [ Ссылка ]. The U87 cell line is crucial to investigate the toxicity of chemotherapies aimed at cancer as well as for studying the in vitro model of glioblastoma cells. Stage 4 glioblastoma or glioblastoma multiform (GBM) is a deadly brain cancer; it is a primary malignant brain tumor, and there are approximately 16,000 new diagnoses yearly in the United States alone. Furthermore, GBM only has a 29.6 % one-year survival rate. The U87 cell line was established from a 44-year old Caucasian female with Stage 3 GBM. The cell line has epithelial morphology and has a modal chromosome number of 44. All of the U87 cells have 12 makers in common and N1, N6 and N9 were not found.
-Altogen Biosystems offers the U87 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The U87 Transfection Reagent is an advanced formulation of a lipid-based reagent, and it has been developed to provide high transfection efficiency with the U87 cell line.
This cell line is used for researching cytotoxicity of chemotherapies as well as for researching glioblastoma. When cultured in vitro, U87 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase U87 Transfection Kit at [ Ссылка ]
-U87 Transfection protocol:
1. Plate 10,000 - 15,000 U87 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with U87 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection.
If the viability of U87 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant.
-General Lipoplex-mediated Transfection Mechanism of Action.
-U87 Transfection Kit Product details:
Two-component formulation enhances lipid-mediated transfection efficiency
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Transfection kit includes Transfection Enhancer reagent
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems ([ Ссылка ])
-Data demonstrates over 80% mRNA knockdown.
-Protein expression of GAPDH in U87 cells.
-U87 Transfection kit benefits:
Pre-optimized transfection protocol for U87 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use U87 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable U87 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
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