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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins - a 2 minute Preview of the Experimental Protocol
Yoo Jung Choi, Jinhui Zhu, Steve Chung, Naveed Siddiqui, Qingping Feng, Peter B. Stathopulos
University of Western Ontario, Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry;
Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.
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