Presented At:
2017 Gibco ExpressionWorld

Presented By:
Matthew McKenna - Scientist, high-throughput transient transfectio, Pfizer, Inc.

Speaker Biography:
2000-2001 Genzyme Biosurgery, GMP Culture of autologous chondrocytes for knee replacement surgery
2001-2002 Harvard School of Public Health, Responsible for culturing of Human Airway Smooth Muscle cells for asthma research
2002-2007 ImmunoGen, Responsible for the stable cell line development of DG44CHO cells secreting monoclonal antibodies

Webinar:
A Semi-Automated High-Throughput Transient Expression and Purification Platform using the Expi293 Expression System

Webinar Abstract:
We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stock with an automated DNA purification system, followed by semi-automated transfection/feed/clarification/filtration and a fully-automated purification and buffer exchange, we deliver high quality-protein supporting dozens of projects with an eight-day turnaround time.

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