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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations - a 2 minute Preview of the Experimental Protocol
Dorrelyn P. Martin, Jharna Miya, Julie W. Reeser, Sameek Roychowdhury
The Ohio State University, Department of Internal Medicine, Division of Medical Oncology, Comprehensive Cancer Center; The Ohio State University, Department of Pharmacology;
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
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