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An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector - a 2 minute Preview of the Experimental Protocol

Daniela Benati, Fabienne Cocchiarella, Alessandra Recchia
University of Modena and Reggio Emilia, Centre for Regenerative Medicine, Department of Life Sciences;

This protocol describes a method to achieve stable integration of a gene of interest into the human genome by the transcriptionally regulated Sleeping Beauty system. Preparation of the integration of defective lentiviral vectors, the in vitro transduction of human cells, and the molecular assay on transduced cells are reported.

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