Isolation Of Bacteria
Isolation Of Bacteria | How To Isolate Plant Pathogenic Bacteria
About This Video:
Isolation of bacteria involves various steps – Specimen collection, Preservation and transportation of specimen, Microscopic examination of sample. Various methods used for isolation of bacteria culture methods which includes culture on solid or liquid media and automated system.
What are the methods of isolation of bacteria?
There are two main ways to isolate organisms.
1:Streaking for isolation on an agar plate.
2:The pour plate method.
How do you isolate pathogens from plants?
The tissues must be blotted dry with a sterile paper towel. 3. Prepare culture media on which the isolated fungal or bacterial pathogens will be grown.
How bacteria are isolated from infected plant part?
The infected tissues along with adjacent small unaffected tissue are cut into small pieces (2–5 mm squares) and by using flame-sterilized forceps, they are transferred to sterile petridishes containing 0.1% mercuric chloride solution used for surface sterilization of plant tissues.

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Isolation Of Bacteria| Techniques
Isolation Of Bacteria| Techniques To Identify Bacterial Infection

About This Video:
There are two method use for identify bacteria infection.
1: Ooze test
2: Cross-section test

Isolation of bacteria:
step involve to identify the growth of pathogen
1:sample collection
2: Surface sterilization
3: sample cutting
4: sample washing
5: pouring media
6: transfer sample on petri plate
7: observed growth after 24 hour
8: Identify the growth under microscope

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