Principle
blood has proteins in it, which interfere with glucose estimation...
So these proteins are precipitated with trichloro acetic acid.
The Contents obtained in the form of filtrate are reffered as protein-free filtrate.
The protein free filtrate contains glucose whose concentrate is to be determined.
So for this, we treat protein-free filtrate and glucose solution in equal volumes with o-toluidine reagent .
The reaction causes the formation of A blue-green N-glycosylamine derivative.
Now, The intensity of this complex that is the blue-green derivative is proportional to the amount or u can say the concentration of glucose present.
