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Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography - a 2 minute Preview of the Experimental Protocol
C. Ryan Oliver, Trisha M. Westerhof, Maria G. Castro, Sofia D. Merajver
University of Michigan Ann Arbor, Department of Internal Medicine; University of Michigan Ann Arbor, Rogel Cancer Center; University of Michigan Ann Arbor, Department of Neurosurgery; University of Michigan Ann Arbor, Department of Cell and Developmental Biology;
Here, we present a protocol for preparing and culturing a blood brain barrier metastatic tumor micro-environment and then quantifying its state using confocal imaging and artificial intelligence (machine learning).
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