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Quality-Controlled Sputum Analysis by Flow Cytometry - a 2 minute Preview of the Experimental Protocol

Marcia Grayson, Shao-Chiang Lai, Lydia H. Bederka, Patricia Araujo, Jamila Sanchez, Xavier T. Reveles, Vivienne I. Rebel, Jennifer Rebeles
bioAffinity Technologies,; The University of Texas Health Science Center at San Antonio, Department of Cell Systems & Anatomy;

This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.

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