The DNS method for estimating the concentration of reducing sugars in a sample was originally invented by G. Miller in 1959.
This method tests for the presence of the free carbonyl group in reducing sugars.
One mole of sugar will react with one mole of 3, 5- dinitro salicylic acid.
The absorbance measured using a spectrophotometer is directly proportional to the amount of reducing sugar. Different reducing sugars generally yield different color intensities; thus, it is necessary to calibrate for each sugar.
Oxidation: Aldehyde group to carboxyl group
Reduction: 3, 5-dinitrosalicylic acid to 3-amino, 5-nitrosalicylic acid

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Previous videos on Enzyme:
1. Introduction to Enzyme || Unit-I || Properties of Enzyme || Factors Affecting Enzyme Activity.
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2. Unit-2 || Enzyme-Substrate Interaction || History of Enzyme - Easily Explained.
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3. Enzyme || Unit 3 || Co-factor || Co-enzyme || Prosthetic group || Apoenzyme || Holoenzyme
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4. Nomenclature and Classification of Enzyme with MNEMONIC and EXAMPLES || Unit 4 || Enzyme
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5. Ribozyme || Synzyme || Abzyme || Enzyme Unit-5
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6. What is “enzyme kinetics”? Why do we study enzyme kinetics? Order of the reaction || Enzyme Unit 6
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7. Zero order reaction-Expression, Graphs, Half-life & Numerical Problems || Enzyme Unit-7
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8. First-Order Reaction || Order of Enzyme-Catalyzed Reaction || Enzyme Kinetics || Enzyme 8 ||
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9. First order reaction || Mathematical Problems and their Solutions || Enzyme 9
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10. Second Order Reaction || Enzyme Kinetics || Enzyme Unit 10
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11. Third Order Reaction - Expression, Graphs, Examples, Numerical Problems & Solutions|| Enzyme Unit 11
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12. nth, Fractional and Negative Order Reaction || n greater than 3 Order Reaction | Enzyme Unit 12
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13. Methods of Determining Reaction ORDER || Summary of Zero, First, Second, and Third Order Reaction
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14. Pseudo-order reaction || Enzyme Unit 14
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15. Enzyme Kinetics - In Detail | The Michaelis–Menten Kinetic Model | Numerical | Enzyme Unit 15
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16. Linear plots of the Michaelis–Menten equation | Nonlinear regression kinetics | Enzyme 16
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