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In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts - a 2 minute Preview of the Experimental Protocol
Marie Cross, Maureen Powers
Emory University, Department of Cell Biology;
Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
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