Watch the Full Video at [ Ссылка ].
Production and Targeting of Monovalent Quantum Dots - a 2 minute Preview of the Experimental Protocol
Daeha Seo, Justin Farlow, Kade Southard, Young-wook Jun, Zev J. Gartner
University of California, San Francisco, Department of Otolaryngology; University of California, Berkeley, Department of Chemistry; Lawrence Berkeley National Laboratory, Materials Science Division; University of California, San Francisco, Department of Pharmaceutical Chemistry; University of California, San Francisco, Tetrad Graduate Program; University of California, San Francisco, Center for Systems and Synthetic Biology; University of California, San Francisco, Chemistry and Chemical Biology Graduate Program;
We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: [ Ссылка ]
