The LMB Mass Spectrometry and Proteomics Facility houses a wide range of mass spectrometers enabling biological proteomics and structural proteomics. Biological proteomics involves the identification of proteins and their interactions and the comparative proteomics of cells and tissues. In structural proteomics purified proteins or their complexes are investigated by hydrogen-deuterium exchange or cross-linking mass spectrometry.
In this video Mark describes a peptide centric approach to structural proteomics. In the first part of the talk, he explains the theory, typical workflow and data analysis involved in hydrogen-deuterium exchange mass spectrometry. How this approach can reveal conformation changes is illustrated with examples of LMB projects including a post-translation modification, mechanism of activity, and the effect of membrane binding. In the second part of the talk, Mark shows how chemical crosslinkers can be used as a tool for structural investigation using an example of the mitochondrial complex I.
The Mass Spectrometry and Proteomics Facility is headed by J Mark Skehel.
Read more about the facility here: [ Ссылка ]
You can find more videos from the LMB 2021 Biophysics Techniques Lecture series here:
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Other playlists you might be interested in:
Next Generation Biophysics Symposium 2020 - [ Ссылка ]
Electron Cryo-Microscopy Course 2017 - [ Ссылка ]
RNA Seq Course - [ Ссылка ]
LMB Crystallography Course 2013 - [ Ссылка ]
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About the MRC Laboratory of Molecular Biology (LMB):
The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.
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