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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5 - a 2 minute Preview of the Experimental Protocol
Eric Ehrke-Schulz, Wenli Zhang, Maren Schiwon, Thorsten Bergmann, Manish Solanki, Jing Liu, Philip Boehme, Theo Leitner, Anja Ehrhardt
University Witten/Herdecke, Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health;
A protocol for generation of high-capacity adenoviral vectors lacking all viral coding sequences is presented. Cloning of transgenes contained in the vector genome is based on homing endonucleases. Virus amplification in producer cells grown as adherent cells and in suspension relies on a helper virus providing viral genes in trans.
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