Learn about using synthetic guide RNAs in your CRISPR experiments. Synthetic sgRNA and crRNA:tracrRNA cause efficient knockout of the targeted proteins as shown by the phenotypic assays and sequence analysis
• Synthetic sgRNA and crRNA:tracrRNA show similar functionality in U2OS and primary CD4+ T cells
• Pooling of the synthetic guide RNAs (sgRNA or crRNA:tracrRNA) ensures robust functional gene
knockout, simplifying loss-of-function studies and screening
