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Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells - a 2 minute Preview of the Experimental Protocol

Andrei A. Kramerov, Mehrnoosh Saghizadeh, Alexander V. Ljubimov
Cedars-Sinai Medical Center, Eye Program, Board of Governors Regenerative Medicine Institute, Departments of Biomedical Sciences and Neurosurgery; University of California, Los Angeles, David Geffen School of Medicine;

An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.

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