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Analyzing Dendritic Morphology in Columns and Layers - a 2 minute Preview of the Experimental Protocol
Chun-Yuan Ting, Philip G. McQueen, Nishith Pandya, Evan S. McCreedy, Matthew McAuliffe, Chi-Hon Lee
National Institutes of Health (NIH), Section on Neuronal Connectivity, Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institutes of Health (NIH), Mathematical and Statistical Computing Laboratory, Center for Information Technology; National Institutes of Health (NIH), Biomedical Imaging Research Services Section, Center for Information Technology;
Here, we show how to analyze dendritic routing of Drosophila medulla neurons in columns and layers. The workflow includes a dual-view imaging technique to improve the image quality and computational tools for tracing, registering dendritic arbors to the reference column array and for analyzing the dendritic structures in 3D space.
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