In this video, I look at the DNA libraries used in molecular biology research.
👉 Cell Biology Playlist - [ Ссылка ]
In the video, I explain the two main types of DNA libraries: genomic DNA (gDNA) libraries and complementary DNA (cDNA) libraries. While both involve cloning DNA sequences into vectors, they serve very different purposes and have unique characteristics.
A gDNA library contains all the DNA from an organism's genome, making it useful for studying gene structure and regulation. However, if derived from eukaryotes it includes both introns and exons, which can complicate specific applications, like protein expression.
A cDNA library is derived from messenger RNA (mRNA) and represents only the genes being actively expressed at the time of collection. This type of library is ideal for studying gene expression and producing proteins but comes with challenges due to its dependency on the cell's mRNA and the challenges of avoiding RNA degradation during preparation.
Resources:
- 🌎 - A help sheet defining key terms is also available in the description - [ Ссылка ]
- 📗 - The Biosciences Glossary available on Google Play Book Store - [ Ссылка ]
- 📗 - Biochemistry (Stryer) - [ Ссылка ] (affiliate link)
- 📗 - Principles of Biochemistry (Lehninger) - [ Ссылка ] (affiliate link)
- 📗 - Molecular Biology of the Cell (Alberts) - [ Ссылка ] (affiliate link)
- 📗 - Molecular Cell Biology (Lodish) - [ Ссылка ] (affiliate link)
- 🫖 - If you would like to say thanks for the video, then please feel free to Buy me a coffee - [ Ссылка ]
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