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Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device - a 2 minute Preview of the Experimental Protocol

Kevin Wong, Angel Ayuso-Sacido, Patrick Ahyow, Andrew Darling, John A. Boockvar, Mingming Wu
Cornell University, Biomedical Engineering Department; Weill Cornell Medical College of Cornell University, Neurosurgical Laboratory for Translational Stem Cell Research, Weill Cornell Brain Tumor Center; Instituto de Investigacion Principe Felipe, Cell Morphology Department; Cornell University, Department of Chemical and Biomolecular Engineering;

We demonstrate that the over expression of epidermal growth factor receptors (EGFR) enhances the motility of neural stem cells(NSCs) using a novel agarose gel based microfluidic device. This technology can be readily adaptable to other mammalian cell systems where cell sources are scarce, such as human neural stem cells, and the turn around time is critical.

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