Light Microscopy - Fundamental Principles - Live Cell Imaging
Learning Objectives:
- Sample preparation
- Choosing a microscope
- Maintaining live cells on a microscope stage
- Efficiency of detection
- Photobleaching and Phototoxicity
- The ultimate limiting factor: Photon Budget
Picture Credits:
Slide 5: "Sample preparation: mounting options"
on the right middle and bottom respectively
1. Cell Chamber: airekacells.com
2. 8-well dark wall slide Cell Culture Slide: Greiner Bio-One
Slide 6: "Sample preparation: mounting options, perfusion systems, microfluidics setup"
A group of 3 images on the top left, from left to right to bottom:
1. www.warneronline.com
2. Ibidi
3. www.elveflow.com
