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Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System - a 2 minute Preview of the Experimental Protocol
Yukie Akutsu, Motomichi Doi, Koji Furukawa, Yuko Takagi
National Institute of Advanced Industrial Science and Technology (AIST), Biomedical Research Institute;
Here, we describe a protocol to introduce a gene knockout into the extracellular amastigote of Trypanosoma cruzi, using the CRISPR/Cas9 system. The growth phenotype can be followed up either by cell counting of axenic amastigote culture or by proliferation of intracellular amastigotes after host cell invasion.
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