Cryogenic electron microscopy (cryo-EM) is a technique where small sample volumes are rapidly frozen at ultra-low temperatures to preserve them in a layer of glass-like ice. The perfectly preserved sample is then imaged using an electron microscope operated at cryogenic temperatures. Cryo-EM provides high-resolution snapshots of the biological building blocks that assemble to form nucleic acids, proteins, viruses, organelles, and cells. In this talk, I will describe how we develop and use cryo-EM technologies to explore the inner-workings of viruses and cells with close to atomic level detail.

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