The video aims to teach student the technique of identifying and separating protein by Immunoblotting (western blotting) gel electrophoresis.
Watch the full video at [ Ссылка ], no sign up needed for open access content, or request a trial to get instant access to our full library.
The video demonstrates step by step procedures like protein separation, blotting the protein in the membrane, immunoprobing and visualization by chromogenic and chemiluminescent substance.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard and the University of Cambridge, benefit from using JoVE’s extensive library of 14000 videos in their research and teaching.
Follow JoVE on Twitter: [ Ссылка ]
Like JoVE on Facebook: [ Ссылка ]
Subscribe to our channel: [ Ссылка ]
Immunoblot Analysis
Теги
ElectrophoresisProteinsAntibodiesTwo Dimentional GelsAntigensProtein markersImmunoprobingBlocking BuffersGel SandwitchProtein BandsImmunostainingelectroblotting tankOrbital ShakerBufferFilter paperPVDF membraneSYPRO RubyCoomassie BlueTTBS and TBS FilterAlkaline PhosphataseAvitin-biotin ComplexPeroxidaseChemiluminescent substanceDAB/NiCl2 TMBBiochemistryEducationResearchScienceLab TechniquesProtein SeparationImmunoblotting