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Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging - a 2 minute Preview of the Experimental Protocol
Helmar Waiczies, Martin Guenther, Julia Skodowski, Stefano Lepore, Andreas Pohlmann, Thoralf Niendorf, Sonia Waiczies
A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, Experimental and Clinical Research Center; Max Delbrück Center for Molecular Medicine, Berlin Ultrahigh Field Facility (B.U.F.F.);
Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.
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