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Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells - a 2 minute Preview of the Experimental Protocol

Melissa V. Fernandez, Elizabeth A. Miller, Nina Bhardwaj
New York University School of Medicine, Department of Pathology; Mount Sinai Medical Center, Division of Infectious Diseases, Department of Medicine; Mount Sinai Medical Center, Division of Hematology and Oncology, Hess Center for Science and Medicine;

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.

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