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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans - a 2 minute Preview of the Experimental Protocol
Barbara Squiban, Jérôme Belougne, Jonathan Ewbank, Olivier Zugasti
Université de la Méditerranée, Centre d’Immunologie de Marseille-Luminy;
We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.
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