Puromycin is really cool. You can use it for selection or to protein-label! I don’t have text written but I just learned about how it works and thought it was cool… blog form: [ Ссылка ]
Here’s a good review article: Aviner R. The science of puromycin: From studies of ribosome function to applications in biotechnology. Comput Struct Biotechnol J. 2020;18:1074-1083. Published 2020 Apr 24. [ Ссылка ]
and here’s the paper on QTI-seq, which uses puromycin to identify translation initiation sites: Gao, X., Wan, J., Liu, B. et al. Quantitative profiling of initiating ribosomes in vivo. Nat Methods 12, 147–153 (2015). [ Ссылка ]
More on ribosome profiling (aka ribosome footprinting aka RiboSeq) here: [ Ссылка ]
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 [ Ссылка ] or search blog: [ Ссылка ]
