Featured Speakers: Professor Manfred Radmacher, University of Bremen and Andrea Slade, Bruker
Cellular Mechanics is attracting an increasing interest in the biophysical and biomedical community. Two main reasons are: (1) the evidence is growing that cell mechanics is a marker of the state of a cell and consequently also the state of a disease at a cellular level, and (2) the availability of AFM allowing to measure quantitatively with high spatial resolution mechanics of cells, e.g. by force curves. Although cells are highly viscous, which is apparent in force curves by a large separation of loading and unloading curve, in most cases this is neglected during analysis of force data, since there is no simple and general way to consider it.
(3:30)
Bruker Applications Scientists, Andrea Slade and Bede Pittenger, will also discuss the latest advances in Bruker’s Bioscope Resolve AFM, including the newly released Ramp Scripting, that are enabling these types of quantitative measurements of the nanomechanical properties of living cells.
(20:46)
Manfred Radmacher, Professor of Biophysics at the University of Bremen Germany will present his latest research showing that the creep response of cells can be measured and analyzed quantitatively when applying a fast step in force or - alternatively - by changing the sample height. With the help of an adequate model sample viscoelastic properties can be derived quantitatively. The data obtained by AFM match whole cell data measured using pipette aspiration. Typical values of viscoelastic properties for adherent cells are elastic moduli around 1kPa, dynamic viscosities around 100 Pa, and relaxation times around 100ms. The latter will determine the timescale at which (whole cell) active processes like shape changes or migration can occur.
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