We show in this video how to prepare the graft Descemet Membrane (DM) in order to perform Descemet Membrane Endothelial Keratoplasty (DMEK) surgery. Although the eye bank may prepare the donor tissue, sometimes the surgeon must prepare and harvest the DM himself. Choosing the right size for the donor punch by measuring the patient's white-to-white diameter before the surgery is essential.

0:00 Intro
0:13 Using the vacuum punch
0:24 Staining with Trypan blue
0:38 First trephination 9.5 mm
0:57 Second trephination 8.0 mm
1:23 Peripheral DM scoring and dissection
2:44 Peripheral tags removal using forceps
3:12 DM graft peeling
4:36 loading the harvested DM into a glass tube.

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#DMEK #graft_preparation #corneal_transplantation #cornea #ophthalmology

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