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Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples - a 2 minute Preview of the Experimental Protocol

Jennifer A. Juno, Genevieve Boily-Larouche, Julie Lajoie, Keith R. Fowke
University of Manitoba, Department of Medical Microbiology; University of Manitoba, Department of Community Health Sciences;

The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.

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