Watch the Full Video at [ Ссылка ].

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate - a 2 minute Preview of the Experimental Protocol

Makoto Kondo, Takamichi Izumi, Nao Fujieda, Atsushi Kondo, Takeharu Morishita, Hirokazu Matsushita, Kazuhiro Kakimi
University of Tokyo Hospital, Department of Immunotherapeutics (Medinet); MEDINET Co., Ltd,;

A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.

Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel: [ Ссылка ]

свернуть