In this video tutorial, I will explain how to optimize the analysis of biophysical properties of membrane-bound vesicles
Content of this video:
0:00 - Intro
0:28 - Synthetic vesicles in VISION
2:41 - Optimize masking of membrane
5:00 - Selection of channel(s) for thresholding
6:06 - Compression function
9:32 - Run the analysis and visualize results
10:50 - Optimize mask for membrane profiling
12:58 - Adherent cells in VISION
14:28 - Membrane masking
16:31 - Cytosol masking
19:04 - Visualizing the results
