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00:00 | Introduction
00:10 | Definition of Recombinant DNA Technology, or rDNA technology
00:43 | Summary of steps in rDNA technology
01:25 | Step 1: identification and isolation of gene of interest From where we get our gene of interest?
02:15 | Step 2: Insertion of this isolated gene in a suitable vector using restriction enzyme and ligase.
02:26 | What is a gene cloning vector? What is called rDNA molecule?
04:10 | Step 3: Introduction of this vector into a suitable organism or cell called the host (transformation)
Gene transfer methods: Physical and chemical gene transfer methods
05:23 | Step 4: Selection of the transformed host cell
06:35 | Step 5: Multiplication or expression of the introduced gene in the host
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