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A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster - a 2 minute Preview of the Experimental Protocol
Timothy P. Murphy, Dan D. Luu, Jessica A. DeSimone, Thomas C. O'Brien, Christopher J. Lally, Jillian J. Lindblad, Sarah M. Webster
College of the Holy Cross, Department of Biology; Georgetown University, School of Medicine; Giesel School of Medicine, Dartmouth College, Department of Biochemistry; Tufts University, School of Medicine; Transgenomic Inc.,; UMass Medical School, Department of Molecular, Cell and Cancer Biology;
In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.
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